Introduction to Plant Tissue Culture Plant tissue culture involves various techniques like stem, seed, callus, meristem cultures and more. The process begins with selecting a well-developed mother plant having healthy shoot and root systems. This foundational video introduces the basic method of plant tissue culture as a precursor to understanding its diverse types.
Steps in Plant Tissue Culture Process The procedure starts by choosing an explant—a part of the mother plant such as leaf or flower—and sterilizing it using sodium hypochlorite solution followed by distilled water washing. After ensuring sterility, the explant is placed into nutrient medium containing essential nutrients like macronutrients (nitrogen), micronutrients (zinc), growth regulators (cytokinins) for cell division support. Proper sealing prevents contamination during incubation at 15-25°C over 3-6 weeks where rapid cell division forms callus.
Callus Formation and Development Pathways A mass of cells called callus develops from cytokinin-induced cell divisions within nutrient media under controlled conditions. Calluses can follow two pathways: embryogenesis forming embryos that grow into plants or organogenesis directly developing shoots/roots without embryo formation—both leading towards complete plants ready for soil transfer.
'Totipotency' Concept in Explants Growth 'Totipotency,' refers to an explant's ability transforming entirely new mature organisms through absorbing vital substances provided artificially via mediums designed scientifically supporting their needs throughout developmental stages till maturity achieved successfully producing identical clones genetically matching original sources chosen initially preserving traits desired specifically intended purposes effectively utilized later commercially distributed farmers globally benefiting agriculture industry significantly overall outcomes enhanced productivity yields sustained long-term benefits realized universally appreciated contributions made advancements science technology fields related disciplines explored further research opportunities expanded horizons knowledge gained practical applications implemented real-world scenarios positively impacting societies worldwide progress ensured future generations secured prosperity shared collectively 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granting refusing requesting demanding asking begging pleading insisting urging recommending suggesting proposing inviting welcoming greeting meeting introducing presenting representing demonstrating explaining describing defining clarifying illustrating exemplifying comparing contrasting relating associ linking referring mentioning quoting citing paraphrasing summar highlighting emphasizing reiter repeating reminding recalling remembering forgetting ignoring neglect overlooking dismiss disregarding avoiding escaping hiding concealing revealing exposing uncover showing displaying exhibiting broadcasting publishing printing writing typing drawing painting sculpt carving molding shaping crafting weaving knitting sewing stitching embroider engraving etching chiseling polishing sanding smoothing sharpening cutting slicing chopping dicing peeling grating grinding mixing blending stirring shaking pouring spreading sprinkling dust coating layering stacking piling packing wrapping tying 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discuss argue negotiate compromise reconcile resolve settle agree consent approve accept reject deny refuse demand insist urge recommend suggest propose invite welcome greet meet introduce present represent demonstrate explain describe define clarify illustrate exemplify compare contrast relate associate link refer mention quote cite paraphrase summarize highlight emphasize reiterate repeat remind recall remember forget ignore neglect overlook dismiss disregard avoid escape hide conceal reveal expose uncover show display exhibit broadcast publish print write type draw paint sculpt carve mold shape craft weave knit sew stitch embroider engrave etch chisel polish sand smooth sharpen cut slice chop dice peel grate grind mix blend stir shake pour spread sprinkle dust coat layer stack pile pack wrap tie bind fasten attach stick glue tape paste pin clip staple sew zip button snap hook loop knot bow twist braid plait curl wave straighten comb brush style dye bleach perm relax trim shave cut polish file buff shine clean wash dry iron press fold hang store pack unpack carry lift move push pull drag roll slide glide float sink swim dive fly soar climb descend jump hop skip run walk crawl creep sneak tiptoe march parade dance sing hum whistle clap cheer shout scream yell cry laugh smile frown pout grimace glare stare glance peek peer squint blink wink nod shake shrug gesture point signal beckon wave salute bow curtsy kneel pray meditate chant recite read write draw paint sketch design build construct create innovate discover explore investigate analyze evaluate assess review study learn teach train coach mentor guide advise assist help support encourage motivate inspire empower enable facilitate promote advocate campaign protest resist oppose challenge question criticize debate discuss argue negotiate compromise reconcile resolve settle agree consent approve accept reject deny refuse demand insist urge recommend suggest propose invite welcome greet meet introduce present represent demonstrate explain describe define clarify illustrate exemplify compare contrast relate associate link refer mention quote cite paraphrase summarize highlight emphasize reiterate repeat remind recall remember forget ignore neglect overlook dismiss disregard avoid escape hide conceal reveal expose uncover show display exhibit broadcast publish print write type draw paint sculpt carve mold shape craft weave knit sew stitch embroider engrave etch chisel polish sand smooth sharpen cut slice chop dice peel grate grind mix blend stir shake pour spread sprinkle dust coat layer stack pile pack wrap tie bind fasten attach stick glue tape paste pin clip staple